Regulatory
Part:BBa_K5280401
Designed by: Hua XU Group: iGEM24_HKUST-GZ (2024-09-07)
PcolE408
PcolE48 is a promoter to which LexRO (BBa_K5280129) binds and represses the expression of genes driven by the promoter. The promoter mainly consists of two parts: ColE promoter, which is a strong promoter, and LexA(408)-operator, which is the binding site of LexRO.
Sequence and Features
Assembly Compatibility:
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21COMPATIBLE WITH RFC[21]
- 23COMPATIBLE WITH RFC[23]
- 25COMPATIBLE WITH RFC[25]
- 1000COMPATIBLE WITH RFC[1000]
Usage and Biology
PcolE408 basically has 2 parts:
- ColE promoter, which is used to drive the expression of downstream genes;
- LexA(408) operator, which is used to provide the regulatory site for LexRO.
The basic working principle is illustrated in the figure below. For more information, you can refer to the part page BBa_K5280129.
This figure shows the mechanism of the eLightOn system (LexRO based regulation system).
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Categories
Parameters
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